top of page

Petri Dish Colonized with Psilocybe Cubensis Amazonian



As a species considered secondary decomposer, the mushroom  Psilocybe cubensis  it can be grown on composted substrate, on coconut fiber, on straw and many other types of substrate (less sawdust, as it does not have many enzymes that degrade lignin).

The final substrate best suited for indoor growing is a mixture of 1 part coconut fiber powder and 1 part fine vermiculite. In addition, add 20% of the initial weight with wheat bran, 2% calcium carbonate and 2% gypsum powder.

Humidity must be corrected to 60-65%, and the substrate must be sterilized within  grow bags with filter  in an autoclave or pressure cooker. After cooling, inoculate the sterile substrate with 10% of “spawn” (seed) previously ready or with  liquid culture. This process should preferably be carried out in a clean place, preferably using a  laminar flow  to avoid contamination. After inoculation, substrate should be incubated at temperatures of 20 to 25ºC.

After colonization, the substrate must be taken to the fruiting room or terrarium, then kept between 20 and 25ºC. After a few days the mushrooms start to grow, reaching up to 5 harvest streams, spaced 3 to 5 days between them.




Found naturally in grasslands in tropical and subtropical regions around the world, this species of mushroom grows in cow dung during the warmest times of the year, typically between spring and autumn.  Indoor cultivation is relatively easy, as the  P. cubensis  grows on a wide variety of substrates that are easily found in garden stores. Considered toxic, consumption of this species is not recommended.


Psilocybe cubensis , known in Brazil as “magic mushrooms”, is a very common mushroom in pastures located in tropical and subtropical zones. The natural substrate of cubensis is cow dung, being considered a saprophytic fungus. In some places it also grows in the dung of elephant, horse and other animals that feed on grasses. It is often found at the hottest times of the year, after several days of rain and sun.

Several strains of this mushroom are cultivated worldwide by collectors. Some of these strains were obtained directly from the fungus growing in its natural habitat, others were obtained through crossbreeding, cloning and genetic selection. Many of these strains have satisfactory biological efficiency, and each has its own phenotypic characteristic. Some are dark, some leucistic, some are completely albino. Some are big and hollow, others are short and massive. The wide variety of  Psilocybe cubensis  allows the grower to explore different strains with different colors, for hobby and/or study purposes.

Colonized Plaque - Psilocybe Cubensis Amazonian

    bottom of page